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All Journal Jurnal Penelitian Saintek Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA JURNAL KIMIA SAINS DAN APLIKASI
Nies Suci Mulyani
Chemistry Department, Faculty Of Sciences And Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Engineering Mathematics

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Journal : SAINS DAN MATEMATIKA

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Biodegradasi Jerami Padi Menggunakan Kompos Termofilik dan Profil SSCP Konsorsium Mikroba Niron, Christina Elsa; Mulyani, Nies Suci; Aminin, Agustina L. N.
JURNAL SAINS DAN MATEMATIKA Volume 20 Issue 2 Year 2012
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Jerami merupakan bahan lignoselulosa, terdiri atas lignin yang terikat pada hemiselulosa dan selulosa. Sebagian besar penelitian biodegradasi biomassa secara mikrobiologis, memanfaatkan kerja mikroba tunggal yang umumnya bekerja kurang optimal. Dalam penelitian ini jerami didegradasi menjadi gula pereduksi menggunakan konsorsium mikroba dari kompos termofilik. Fermentasi jerami padi dilakukan pada media semisolid menggunakan starter kompos termofilik, dan proses degradasi dipantau berdasarkan kadar gula pereduksi yang dihasilkan setiap 24 jam. Kelimpahan mikroba diamati melalui fragmen gen 16S dan 18S rRNA dengan teknik SSCP (Single Strand Conformation Polymorphism). Degradasi optimum jerami padi didapatkan pada jam ke-72 dengan kadar gula sebesar 0,474 mg/L. Profil pita-pita SSCP ditunjukkan dengan perbedaan jumlah dan intensitas pita-pita dari kelompok jamur maupun bakteri. Perbedaan pita-pita tersebut menunjukkan adanya perbedaan kelimpahan komunitas mikroba baik pada konsorsium kompos maupu mikroba pendegradasi jerami padi.   Keywords: biodegradasi, kompos termofilik, komunitas mikroba, SSCP
16S rRNA Gene Analysis of Chlorate Reducing Thermophilic Bacteria from Local Hot Spring Aminin, Agustina L. N.; Katulistiwasari, Puri; Mulyani, Nies Suci
JURNAL SAINS DAN MATEMATIKA Volume 19 Issue 4 Year 2011
Publisher : JURNAL SAINS DAN MATEMATIKA

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Chlorates waste remediation by biological processes has been the object of current research. Strain CR, the chlorate reducing bacteria was isolated from Gedongsongo hot spring using minimal medium broth containing chlorates and acetate at 55oC. The determination of chlorate reduction from medium was carried out using turbidimetric method. CR isolate showed reducing ability 18% after four days of incubation. The phenotypic character of CR isolate including rod-shaped cells, gram-positive bacteria and facultative anaerobes. On the basis of 16S rRNA analysis, CR isolate was closely related to Bacillus pallidus, Aeribacillus E3, Geobacillus pallidus and Geobacillus sp.D64. The similarity of nucleotide sequences of 16S rRNA gene was 97%, suggested as a novel species.   Keyword: thermophilic bacteria, chlorate reducing, 16S rRNA gene
Co-Authors Agustina L.N. Aminin Asy'ari, Mukhammad Christina Elsa Niron Didik Setiyo Widodo Dini Kurnia Wisatya Dwi Hudiyanti Enny Fachriyah Heru Prasetiyoningsih Ina Noprastika Ismiyarto Ismiyarto Ken Ima Damayanti Mukhammad Asy’ari Ngadiwiyana M.Si. S.Si. Nor Basid Adiwibawa Prasetya Okky Triana Purbowatiningrum Ria Sarjono Purbowatiningrum Ria Sarjono Puri Katulistiwasari Sandi Sutopo Aribowo Siti Anisa Sumardjo, Damin Ulfa Wiwit Chasanah Wirnia Sinar Setyani Wuryanti Wuryanti Yanidya Tanjihah Ardiansyah Yuga Pratama